[article]
| Titre : |
Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum : a propagation protocol for an endangered Florida native orchid |
| Type de document : |
texte imprimé |
| Auteurs : |
Daniela Dutra, Auteur ; Michael Kane, Auteur ; Larry Richardson, Auteur |
| Année de publication : |
2009 |
| Article en page(s) : |
pp. 235-243 |
| Langues : |
Anglais (eng) |
| Catégories : |
[Espèces (in biblio)] Orchidaceae
|
| Mots-clés : |
graine germination conservation |
| Résumé : |
"Cyrtopodium punctatum Lindley is an endangered epiphytic orchid restricted in the United States to southern Florida. Due to its ornamental value, the species was extensively collected from the wild during the past 100 years. Today, only a few plants remain in protected areas. As part of a conservation plan for the species, procedures for asymbiotic seed germination were developed. Five asymbiotic orchid seed germination media (PhytoTechnology Orchid Seed Sowing Medium, Knudson C, Malmgren Modified Terrestrial Orchid Medium, Vacin & Went Modified Orchid Medium, and ½-strengh Murashige & Skoog) were examined for their effectiveness in promoting seed germination and protocorm development under a 16/8 h L/D photoperiod and dark (0/24 h L/D). The influence of photoperiod on growth and development was also examined. Seeds were germinated under a 16/8 h, 12/12 h, 8/16 h L/D photoperiod, at 25 ± 3°C and allowed to develop in vitro for 10 weeks. After 10 weeks, developing seedlings were transferred to Sigma Phytatrays and returned to their assigned photoperiod treatments for continued seedling development for an additional 15 weeks. Highest germination occurred in 0/24 h L/D on PhytoTechnology Orchid Seed Sowing Medium and seedlings displayed more advanced development when cultured under 16/8 h L/D photoperiod after 15 weeks in Phytatrays. Thirty-five week old seedlings potted in coconut husk growing medium exhibited 90% survival following 5 weeks acclimatization to greenhouse conditions. This asymbiotic seed germination protocol for C. punctatum will facilitate future reintroduction projects involving this endangered species." (source : auteurs) |
| Type de publication : |
périodique |
| Référence biblio : |
Dutra D., Kane M., Richardson L., 2009 - Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum : a propagation protocol for an endangered Florida native orchid. Plant Cell, Tissue and Organ Culture, 96 (3) : 235-243. |
| ID PMB : |
64130 |
| Permalink : |
http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=64130 |
in Plant Cell, Tissue and Organ Culture > vol. 96, n°3 (Mars 2009) . - pp. 235-243
[article]
|
Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum : a propagation protocol for an endangered Florida native orchid in Plant Cell, Tissue and Organ Culture, vol. 96, n°3 (Mars 2009)