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A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A / Wojciech J. Szypula in Acta societatis botanicorum poloniae, vol. 82, n°4 (Année 2013)  

Public [article]  inActa societatis botanicorum poloniae > vol. 82, n°4 (Année 2013) . - pp. 313-320 Titre : A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A Type de document : texte imprimé Auteurs : Wojciech J. Szypula, Auteur ; Paulina Mistrzak, Auteur ; Olga Olszowska, Auteur Année de publication : 2013 Article en page(s) : pp. 313-320 Langues : Anglais (eng) Catégories : [Espèces (in biblio)] Huperzia selago Mots-clés : régénération  protocole  culture in-vitro Résumé : "This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some neurodegenerative diseases. The proposed procedure allowed approximately tenfold shortening of the species developmental stages with the omission of the gametophyte stage while the sporophyte mass could be increased tenfold within a 6-month period. The cultures were established using vegetative propagules (bulbils) procured from sporophytes growing in the wild without degrading the habitats of this endangered plant species. Explants underwent surface and internal disinfection to eliminate the epiphytic and endophytic bacteria and fungi. In in vitro cultures, the optimum results were achieved using Moore (Mr) medium without growth regulators or supplemented with 0.015 mg/l IBA and 0.3 mg/l kinetin. These media ensured both viability of the propagules and their further development. The biomass growth index for H. selago sporophytes grown from propagules, determined at 3 months of culture (1 passage) on Mr medium with IBA and kinetin was 650%. At 6 months, the biomass growth index increased to 1114%. Vigorous growth of adventitious roots, especially on Mr medium with the addition of 0.25 mg/l NAA, and callus formation on shoot apices were observed. At 6 months of culture, some sporophytes obtained from the bulbils were used as the initiating material for shoot subcultures, which developed best on Mr medium with IBA and kinetin." (source : auteurs) Type de publication : périodique Référence biblio : Szypula W., Mistrzak P., Olszowska O., 2013 - A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A. Acta societatis botanicorum poloniae, 82 (4) : 313-320. ID PMB : 61126 En ligne : https://pbsociety.org.pl/journals/index.php/asbp/article/view/asbp.2013.034 Format de la ressource électronique : document Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=61126 [article]

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Somatic embryogenesis and in vitro culture of Huperzia selago shoots as a potential source of huperzine A / Wojciech J. Szypula in Plant Science, vol. 168, n°6 (Juin 2005)

Public [article]  inPlant Science > vol. 168, n°6 (Juin 2005) . - pp. 1443-1452 Titre : Somatic embryogenesis and in vitro culture of Huperzia selago shoots as a potential source of huperzine A Type de document : texte imprimé Auteurs : Wojciech J. Szypula, Auteur ; Agnieszka Pietrosiuk, Auteur ; Piotr Suchocki, Auteur ; Olga Olszowska, Auteur ; Mirosława Furmanowa, Auteur ; Olga Kazimierska, Auteur Année de publication : 2005 Article en page(s) : pp. 1443-1452 Langues : Anglais (eng) Catégories : [Espèces (in biblio)] Huperzia selago [Espèces (in biblio)] Lycopodiaceae Mots-clés : reproduction  culture in vitro  embryogénèse  Huperzine A Résumé : "In vitro culture of the sporophytes and somatic embryogenesis in Huperzia selago is described together with the results of phytochemical analyses to determine the huperzine A content of H. selago shoots collected from plants growing in vitro and in a natural habitat. The in vitro culture was initiated from shoots of wild plants, which underwent surface and internal sterilization to eliminate the epiphytic and endophytic bacteria and fungi and were next placed on different modifications of the Murashige and Skoog medium (MS). The highest growth frequency was achieved on the MS medium at half-strength mineral salt content (1/2 MS). The same medium was used for the induction of somatic embryogenesis. Cells of the callus, which developed from the apical meristem after 3 months of incubation, transformed into somatic embryos. The stages of their further growth resembled the zygotic embryos of other Lycopodiaceae. The developing embryo had no shoot–root axis, but one that corresponded to the shoot–foot axis with the root growing at its side. The developing somatic embryo smoothly transformed into a young sporophyte with pre-leaves and numerous proper leaves and an embryonal root. HPLC–UV assay demonstrated the highest huperzine A content (3.33 mg g−1 d.w.) in the shoots of plants grown from the somatic embryos. The yield of huperzine A from plants collected from a natural habitat differed from 0.54 (shoots harvested in spring) to 1.27 mg g−1 d.w. (shoots harvested in autumn)." (source : auteurs) Type de publication : périodique Référence biblio : Szypula W., Pietrosiuk A., Suchocki P., Olszowska O., Furmanowa M., Kazimierska O., 2005 - Somatic embryogenesis and in vitro culture of Huperzia selago shoots as a potential source of huperzine A. Plant Science, 168 (6) : 1443-1452. ID PMB : 64986 Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=64986 [article]

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