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Computer-Assisted Recovery of Threatened Plants : Keys for Breaking Seed Dormancy of Eryngium viviparum / Manuel Ayuso in Frontiers in Plant Science, vol. 8 (Année 2017)  

Public [article]  inFrontiers in Plant Science > vol. 8 (Année 2017) . - pp. e2092 Titre : Computer-Assisted Recovery of Threatened Plants : Keys for Breaking Seed Dormancy of Eryngium viviparum Type de document : texte imprimé Auteurs : Manuel Ayuso, Auteur ; Pablo Ramil, Auteur ; Mariana Landin, Auteur ; Pedro Gallego, Auteur ; Esther Barreal, Auteur Année de publication : 2017 Article en page(s) : pp. e2092 Langues : Anglais (eng) Catégories : [Espèces (in biblio)] Eryngium viviparum Mots-clés : conservation  germination  dormance  graine  plante endémique  taux de germination  intelligence artificielle  modélisation Résumé : "Many endangered plants such as Eryngium viviparum (Apiaceae) present a poor germination rate. This fact could be due to intrinsic and extrinsic seed variability influencing germination and dormancy of seeds. The objective of this study is to better understand the physiological mechanism of seed latency and, through artificial intelligence models, to determine the factors that stimulate germination rates of E. viviparum seeds. This description could be essential to prevent the disappearance of endangered plants. Germination in vitro was carried out under different dormancy breaking and incubation procedures. Percentages of germination, viability and E:S ratio were calculated and seeds were dissected at the end of each assay to describe embryo development. The database obtained was modeled using neurofuzzy logic technology. We have found that the most of Eryngium seeds (62.6%) were non-viable seeds (fully empty or without embryos). Excluding those, we have established the germination conditions to break seed dormancy that allow obtaining a real germination rate of 100%. Advantageously, the best conditions pointed out by neurofuzzy logic model for embryo growth were the combination of 1 mg L?1 GA3 (Gibberellic Acid) and high incubation temperature and for germination the combination of long incubation and short warm stratification periods. Our results suggest that E. viviparum seeds present morphophysiological dormancy, which reduce the rate of germination. The knowledge provided by the neurofuzzy logic model makes possible not just break the physiological component of dormancy, but stimulate the embryo development increasing the rate of germination. Undoubtedly, the strategy developed in this work can be useful to recover other endangered plants by improving their germination rate and uniformity favoring their ex vitro conservation." (source : auteurs) Type de publication : périodique Référence biblio : Ayuso M., Ramil P., Landin M., Gallego P., Barreal E., 2017 - Computer-Assisted Recovery of Threatened Plants : Keys for Breaking Seed Dormancy of Eryngium viviparum. Frontiers in Plant Science, 8 : e2092. ID PMB : 67479 En ligne : https://www.frontiersin.org/articles/10.3389/fpls.2017.02092/full Format de la ressource électronique : document Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=67479 [article]

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In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds / Manuel Ayuso in Plant Cell, Tissue and Organ Culture, vol. 138 (Année 2019)

Public [article]  inPlant Cell, Tissue and Organ Culture > vol. 138 (Année 2019) . - pp. 427-435 Titre : In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds Type de document : texte imprimé Auteurs : Manuel Ayuso, Auteur ; Pascual García-Pérez, Auteur ; Pablo Ramil-Rego, Auteur ; Pedro Gallego, Auteur ; Esther Barreal, Auteur Année de publication : 2019 Article en page(s) : pp. 427-435 Langues : Anglais (eng) Catégories : [Espèces (in biblio)] Eryngium viviparum Mots-clés : plante endémique  plante menacée  conservation  culture in vitro  conservation ex situ Résumé : "Different Eryngium species have been used with ornamental, agricultural and medicinal purposes, as a consequence of their chemical constituents. In the southwest Europe the endemic Eryngium viviparum, presents a high risk of extinction and ex situ strategies are high recommended for efficient conservation and re-introduction program. The objective of this study was to satisfy a dual objective: (i) to develop an ex situ conservation strategy through micropropagation and (ii) taking advantage of the extraordinary potential of plant tissue culture, produce a considerable amount of plant material to carry out a preliminary phytochemical study, based on the accumulation of phenolic compounds and their associated antioxidant activity. First a factorial design was conducted in order to study the effect of two cytokinins (6- benzylaminopurine, BAP, and kinetin, KIN), at three levels (0, 1 and 2 mg L−1), on shoot multiplication. Later another factorial design was applied, by using three levels of MS medium salt strength (full, half and quarter- strength) and four sucrose levels (0, 1, 2, and 3%) for improving shoot elongation and rooting. In parallel, a preliminary quantification of total phenolic and flavonoid contents from E. viviparum aerial parts was determined. The simple micropropagation protocol designed allowed obtaining a high rates of shoot multiplication (5.1–5.8 new shoots), rooting (100%) with healthy long roots (3.1–3.5 cm) and plantlet acclimatization (96%). Moderate antioxidant activity was recorded in hydromethanolic extracts from E. viviparum aerial parts. High correlation between total phenolic content and BAP levels in the culture media was found. In conclusion, the micropropagation procedure described here for the endangered E. viviparum can be used as new and very efficient ex situ conservation strategy, and as potential source of antioxidants, conferring an added-value to this plant." (source : auteurs) Type de publication : périodique Référence biblio : Ayuso M., García-Pérez P., Ramil-Rego P., Gallego P., Barreal E., 2019 - In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds. Plant Cell, Tissue and Organ Culture, 138 : 427-435. ID PMB : 69165 Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=69165 [article]

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