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In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds / Manuel Ayuso in Plant Cell, Tissue and Organ Culture, vol. 138 (Année 2019)

Public [article]  inPlant Cell, Tissue and Organ Culture > vol. 138 (Année 2019) . - pp. 427-435 Titre : In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds Type de document : texte imprimé Auteurs : Manuel Ayuso, Auteur ; Pascual García-Pérez, Auteur ; Pablo Ramil-Rego, Auteur ; Pedro Gallego, Auteur ; Esther Barreal, Auteur Année de publication : 2019 Article en page(s) : pp. 427-435 Langues : Anglais (eng) Catégories : [Espèces (in biblio)] Eryngium viviparum Mots-clés : plante endémique  plante menacée  conservation  culture in vitro  conservation ex situ Résumé : "Different Eryngium species have been used with ornamental, agricultural and medicinal purposes, as a consequence of their chemical constituents. In the southwest Europe the endemic Eryngium viviparum, presents a high risk of extinction and ex situ strategies are high recommended for efficient conservation and re-introduction program. The objective of this study was to satisfy a dual objective: (i) to develop an ex situ conservation strategy through micropropagation and (ii) taking advantage of the extraordinary potential of plant tissue culture, produce a considerable amount of plant material to carry out a preliminary phytochemical study, based on the accumulation of phenolic compounds and their associated antioxidant activity. First a factorial design was conducted in order to study the effect of two cytokinins (6- benzylaminopurine, BAP, and kinetin, KIN), at three levels (0, 1 and 2 mg L−1), on shoot multiplication. Later another factorial design was applied, by using three levels of MS medium salt strength (full, half and quarter- strength) and four sucrose levels (0, 1, 2, and 3%) for improving shoot elongation and rooting. In parallel, a preliminary quantification of total phenolic and flavonoid contents from E. viviparum aerial parts was determined. The simple micropropagation protocol designed allowed obtaining a high rates of shoot multiplication (5.1–5.8 new shoots), rooting (100%) with healthy long roots (3.1–3.5 cm) and plantlet acclimatization (96%). Moderate antioxidant activity was recorded in hydromethanolic extracts from E. viviparum aerial parts. High correlation between total phenolic content and BAP levels in the culture media was found. In conclusion, the micropropagation procedure described here for the endangered E. viviparum can be used as new and very efficient ex situ conservation strategy, and as potential source of antioxidants, conferring an added-value to this plant." (source : auteurs) Type de publication : périodique Référence biblio : Ayuso M., García-Pérez P., Ramil-Rego P., Gallego P., Barreal E., 2019 - In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds. Plant Cell, Tissue and Organ Culture, 138 : 427-435. ID PMB : 69165 Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=69165 [article]

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