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In vitro propagation and shoot encapsulation as tools for ex situ conservation of the aquatic plant Ludwigia palustris (L.) Ell. / L. Fontanili in Plant Biosystems, vol. 149, n°5 (Année 2015)

Public [article]  inPlant Biosystems > vol. 149, n°5 (Année 2015) . - pp. 855-864 Titre : In vitro propagation and shoot encapsulation as tools for ex situ conservation of the aquatic plant Ludwigia palustris (L.) Ell. Type de document : texte imprimé Auteurs : L. Fontanili, Auteur ; M. Lucchesini, Auteur ; A. Mensuali-Sodi, Auteur Année de publication : 2015 Article en page(s) : pp. 855-864 Langues : Anglais (eng) Catégories : [habitats/milieux] 2 - Milieux aquatiques non marins [habitats/milieux] ZH - Zones humides [Thèmes] Banque de graines Mots-clés : conservation ex situ  culture  graine  encapsulation  micropropagation  stockage Résumé : "Ludwigia palustris (L.) Ell. is an aquatic perennial herb present in several regions of Italy, which is one of its native countries. In this research, micropropagation and encapsulation protocols were established from axillary buds of L. palustris. Shoots proliferated on half-strength Murashige and Skoog medium without growth regulators. Different culture vessels were tested. Shoots in GROWTEK bioreactor showed the highest fresh and dry weight and total length while the plantlets grown in the RITA bioreactor showed the highest shoot number per explant. Encapsulation of L. palustris microcuttings with sodium alginate formed small and whitish beads which were stored for 14 or 28 days at 7° or 25°C. Storage for 14 days at both temperatures gave the best results but prolonged storage at 25°C decreased the shoot viability to 73%. After 4 weeks of recovery, all the plantlets showed the typical features of the species. Even though the latest Italian IUCN Red List does not mention L. palustris, conservation measures are proposed at local level because this species locally remains vulnerable mainly due to the loss of adequate habitats.Our protocol could be one of the methods for ex situ conservation of L. palustris particularly because its seed storage behavior is uncertain." (source : auteurs) Type de publication : périodique Référence biblio : Fontanili L., Lucchesini M., Mensuali-Sodi A., 2015 - In vitro propagation and shoot encapsulation as tools for ex situ conservation of the aquatic plant Ludwigia palustris (L.) Ell. Plant Biosystems, 149 (5) : 855-864. ID PMB : 62008 DOI : 10.1080/11263504.2014.911779/10.1080/11263504.2014.911779 Permalink : http://www.cbnbrest.fr/catalogue_en_ligne/index.php?lvl=notice_display&id=62008 [article]

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